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Cell Biology: A Laboratory Handbook, by Julio E Celis
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- Sales Rank: #5493213 in Books
- Published on: 1997
- Original language: English
- Number of items: 1
- Binding: Paperback
Most helpful customer reviews
1 of 1 people found the following review helpful.
Greatest value of this work is its comprehensiveness
By Firochromis
Cell biology techniques have been continuously upgraded since the middle of this century, and future developments seem assured. In the 1990s, alone, new developments have led to improved separations capability, detection instrumentation, and new formulations of serum-free media. Any research institution with faculty and students engaged in cell biology will want to have the most current laboratory guidelines available, and this four-volume, peer-reviewed collection of protocols answers that need. The second edition has benefited from suggestions from practicing scientists, filling in gaps from the first edition.
Perhaps the greatest value of this work is its comprehensiveness. Laboratories and libraries can have detailed guidelines for cell culture, separation, staining, growth, apoptosis, preparation of component parts, assays, antibody production, genetic engineering, microscopy, electrophoresis, and more, all in one source. Need to know how to produce transgenic mice? It's here. How to grow viruses? Prepare nuclei? Stain mitochondria? Use fluorecence microscopy? All here.
Although there are 486 contributors to the 250 articles, uniform structure of articles builds continuity and thus ease of use. Most entries adhere to the five-part template (Introduction, Materials and Instrumentation, Procedures, Comments, and Pitfalls). This last section, "Pitfalls," seems especially valuable, as there is no substitute for experience. The main difference between articles tends to be the use of supportive graphs, photos, and tables, though in general (if such a diverse collection can be spoken of "in general") visuals are at a minimum.
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